Intermediate
Technical
IVT
amplicon sequencing
Targeted sequencing method that amplifies and sequences specific genomic regions of interest in cancer samples.
Full Definition
A focused next-generation sequencing approach that uses PCR amplification to target and sequence predefined genomic regions, typically hotspot mutations in cancer-related genes. This method is commonly used in clinical oncology for rapid, cost-effective analysis of known actionable mutations. Amplicon sequencing provides high depth of coverage for targeted regions but lacks the breadth of whole exome or genome sequencing approaches.
Usage
Usage note: Often confused with broader panel sequencing approaches; specify the targeted nature when editing clinical documents.
In Context
- "The laboratory used amplicon sequencing to detect EGFR mutations in the lung cancer specimen." — Clinical genomics report
- "Amplicon sequencing results were available within 72 hours, enabling rapid treatment decisions." — Molecular pathology report
Also known as
targeted amplicon sequencing
hotspot sequencing
Contrasted with
whole genome sequencing
whole exome sequencing
Don't confuse with
panel sequencing
capture-based sequencing