FFPE
Also written as: FFPE — formalin-fixed, paraffin-embedded
Formalin-fixed, paraffin-embedded tissue samples commonly used for histopathological examination and molecular analysis.
Full Definition
Formalin-fixed, paraffin-embedded (FFPE) tissue represents the standard method for preserving clinical tissue specimens in pathology laboratories. The formalin fixation process cross-links proteins and nucleic acids to preserve tissue morphology, while paraffin embedding provides structural support for sectioning. In cancer genomics, FFPE samples are frequently the only available material for molecular analysis, particularly for retrospective studies or when fresh tissue is unavailable. However, the fixation process can introduce artifacts including DNA fragmentation and cytosine deamination, which must be considered during genomic analysis and variant interpretation.
Usage
Usage note: Spell out on first use; note potential limitations for certain molecular analyses.
In Context
- "DNA extraction from FFPE blocks yielded sufficient material for targeted sequencing analysis." — Laboratory protocol
- "The study included 150 FFPE tumor samples collected over a five-year period." — Research methodology