Enzyme-Linked Immunosorbent Assay
Also written as: ELISA — Enzyme-linked immunosorbent assay
A plate-based immunoassay technique using enzymes as detection markers for hormone measurement.
Full Definition
Enzyme-linked immunosorbent assay (ELISA) is a widely used immunoassay method that employs enzyme-substrate reactions to detect and quantify hormones. The technique involves binding target hormones to specific antibodies attached to a solid surface, followed by detection using enzyme-conjugated secondary antibodies. ELISA offers good precision and is commonly used for research applications and some clinical hormone measurements, though it has largely been replaced by automated platforms in high-volume laboratories.
Usage
Usage note: Spell out on first use, then use 'ELISA'; include hyphens in full form.
In Context
- "The enzyme-linked immunosorbent assay showed good correlation with reference methods." — Method validation study
- "ELISA techniques require careful standardization to ensure reproducible results." — Laboratory quality manual