Hormone Binding Assay
Laboratory method measuring specific hormone-protein interactions to assess binding capacity and free hormone levels.
Full Definition
Hormone binding assays evaluate the interaction between hormones and their binding proteins, providing information about hormone transport, bioavailability, and receptor binding. These assays can measure binding protein concentrations (such as sex hormone-binding globulin or thyroid-binding globulin), binding affinity, or calculate free hormone fractions. Different methodological approaches include equilibrium dialysis, ultrafiltration, and competitive binding techniques. Clinical editors should understand that binding assays complement total hormone measurements by providing information about the biologically active hormone fraction, and that binding protein alterations can significantly affect hormone physiology without changing total hormone levels.
Usage
Usage note: Specify the binding protein and methodology when documenting binding assay results.
In Context
- "Hormone binding assays revealed decreased SHBG capacity in the insulin-resistant patient." — Research study methodology
- "The hormone binding assay protocol requires careful temperature and pH control." — Laboratory procedure manual