Liquid Chromatography-Tandem Mass Spectrometry
Also written as: LC-MS/MS — Liquid Chromatography-Tandem Mass Spectrometry
The gold standard analytical method for hormone testing, providing superior specificity and accuracy through molecular separation and detection.
Full Definition
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) combines chromatographic separation with mass spectrometric detection to provide the most accurate and specific method for hormone analysis. This technique physically separates molecules before detection, eliminating cross-reactivity issues common in immunoassays and providing precise identification based on molecular mass and fragmentation patterns. LC-MS/MS is particularly valuable for measuring steroid hormones, which often have similar structures that can cross-react in immunoassays. The method also enables simultaneous measurement of multiple analytes and can detect metabolites or unusual forms of hormones. While more expensive and technically complex than immunoassays, LC-MS/MS is increasingly used as the reference method for hormone testing and for resolving discordant immunoassay results.
Usage
Usage note: Often considered the gold standard for steroid hormone measurements due to superior specificity.
In Context
- "LC-MS/MS confirmed the testosterone result after immunoassay interference was suspected." — Laboratory correlation study
- "The laboratory implemented liquid chromatography-tandem mass spectrometry for improved steroid hormone accuracy." — Method implementation report