immunohistochemistry
Pronunciation: ih-myoo-noh-his-toh-KEM-is-tree
Also written as: IHC — Immunohistochemistry
Laboratory technique using antibodies to detect specific proteins in tissue sections, essential for neuropathological diagnosis.
Full Definition
Immunohistochemistry (IHC) is a laboratory method that uses specific antibodies to identify and localize proteins within tissue sections. In neuropathology, IHC is crucial for diagnosing brain tumors, neurodegenerative diseases, and inflammatory conditions by detecting characteristic protein markers. Common markers include GFAP for astrocytes, neurofilament for neurons, and various tumor-specific antigens. The technique involves applying primary antibodies followed by detection systems that produce visible signals, typically brown chromogen deposits. Editors must understand IHC terminology when reviewing diagnostic reports and research manuscripts in neuropathology.
Usage
Usage note: Specify antibodies and detection methods when describing procedures; distinguish from other molecular techniques.
In Context
- "Immunohistochemistry for GFAP confirmed the astrocytic lineage of the tumor cells." — Pathology report
- "The diagnosis was supported by positive immunohistochemistry for IDH1-R132H mutation." — Molecular neuropathology report