Professional
Technical
IVT
fluorometric assay
An analytical method using fluorescence detection to measure enzyme activity or substrate concentration.
Full Definition
Fluorometric assays are laboratory techniques that measure the fluorescent properties of compounds to quantify analytes in newborn screening specimens. These assays are commonly used for enzyme activity measurements, such as GALT activity for galactosemia screening, and for detecting specific metabolites. The method offers high sensitivity and specificity, making it suitable for detecting low concentrations of target compounds in small blood volumes. Results are typically expressed as fluorescence units or enzyme activity rates.
Usage
Usage note: Distinguish from fluorescence microscopy, which is a different analytical technique.
In Context
- "The laboratory uses a fluorometric assay to measure biotinidase enzyme activity." — Standard operating procedure
- "Fluorometric assay results showed elevated phenylalanine levels requiring confirmation." — Quality control report
Also known as
fluorescence assay
fluorimetric assay
Contrasted with
colorimetric assay
Don't confuse with
fluorescence polarization
time-resolved fluorescence