Intermediate Technical In the Industry Vocabulary Test

next-generation sequencing

Also written as: NGS — next-generation sequencing

High-throughput DNA sequencing technologies enabling rapid analysis of multiple genes or entire genomes simultaneously.

Full Definition

Next-generation sequencing (NGS) encompasses massively parallel sequencing technologies that can analyze millions of DNA fragments concurrently, enabling comprehensive genomic profiling at unprecedented scale and speed. In oncology informatics, NGS generates vast datasets requiring sophisticated bioinformatics pipelines for variant calling, annotation, and clinical interpretation. Applications range from targeted cancer gene panels to whole-exome and whole-genome sequencing for precision oncology applications.

Usage

Usage note: Hyphenate compound modifier; avoid abbreviation in formal titles.

In Context

"The laboratory implemented next-generation sequencing workflows to replace single-gene testing for hereditary cancer syndromes." — Laboratory operations update
"Next-generation sequencing identified actionable mutations in 34% of patients with advanced solid tumors." — Precision oncology program outcome report

Also known as

NGS massively parallel sequencing high-throughput sequencing

Contrasted with

Sanger sequencing first-generation sequencing

Don't confuse with

third-generation sequencing long-read sequencing

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