flow cytometry
A laboratory technique used to analyze cell populations by measuring physical and chemical characteristics of individual cells.
Full Definition
A powerful analytical technique that uses laser technology to examine multiple characteristics of individual cells simultaneously, including size, complexity, and protein expression patterns. In pediatric immunology, flow cytometry is essential for immunophenotyping, determining absolute cell counts, measuring cell function, and monitoring immune reconstitution. The technique allows for detailed analysis of lymphocyte subsets, identification of immunodeficiency patterns, and assessment of treatment responses. Results are typically presented as percentages and absolute numbers of specific cell populations, requiring careful interpretation in the context of age-appropriate reference ranges.
Usage
Usage note: Often abbreviated as 'flow' in clinical contexts; distinguish from cell sorting applications.
In Context
- "Flow cytometry analysis revealed severely reduced CD4+ T-cell counts consistent with immunodeficiency." — Laboratory report
- "Serial flow cytometry studies monitored immune reconstitution following gene therapy." — Research protocol documentation