allele-specific PCR
Molecular technique that selectively amplifies specific genetic variants to detect point mutations in tumor DNA.
Full Definition
Allele-specific PCR (polymerase chain reaction) is a targeted molecular diagnostic method designed to detect specific nucleotide variations in tumor samples. This technique uses primers that selectively bind to either wild-type or mutant DNA sequences, enabling precise identification of clinically relevant point mutations. The method is particularly valuable for detecting low-frequency mutations in heterogeneous tumor samples and monitoring minimal residual disease. Results from allele-specific PCR inform targeted therapy selection and treatment monitoring in precision oncology protocols.
Usage
Usage note: Hyphenate when used as compound modifier; avoid abbreviating to AS-PCR in patient reports.
In Context
- "Allele-specific PCR detected the EGFR L858R mutation at 15% allelic frequency." — Molecular diagnostics report
- "The laboratory validated allele-specific PCR protocols for routine KRAS testing." — Laboratory procedure manual