fertilization check
The laboratory assessment performed 16-20 hours after insemination or ICSI to confirm successful fertilization by observing pronuclei formation.
Full Definition
A fertilization check is a critical laboratory procedure performed the morning after egg retrieval and insemination to assess whether fertilization has occurred successfully. Embryologists examine each inseminated egg under high magnification to identify the presence of two pronuclei (2PN), which indicates normal fertilization. Abnormal fertilization patterns, such as 0PN (no fertilization), 1PN, or 3PN (polyspermy), are also documented. This assessment determines how many normally fertilized embryos are available for culture and potential transfer, directly impacting treatment success rates and patient counseling.
Usage
Usage note: Document the number of 2PN embryos and any abnormal fertilization patterns observed.
In Context
- "The fertilization check revealed 8 normally fertilized embryos (2PN) out of 12 mature eggs." — Embryology laboratory report
- "Fertilization check results will be communicated to patients by noon today." — Laboratory workflow note