AAV
Pronunciation: A-A-V
Also written as: AAV — Adeno-Associated Virus
A small, non-enveloped single-stranded DNA virus widely used as a delivery vector for CRISPR components and gene therapy payloads due to its low immunogenicity and ability to transduce non-dividing cells.
Full Definition
Adeno-associated virus (AAV) is a member of the Parvoviridae family used extensively in gene therapy and CRISPR research as a delivery vector. Its key advantages include low pathogenicity, broad tissue tropism determined by serotype (e.g., AAV2, AAV5, AAV9), and long-term gene expression in post-mitotic tissues. In CRISPR delivery, AAV is used to package gRNA-encoding sequences or smaller Cas orthologs such as SaCas9. Editors should always define 'AAV' at first use and specify the serotype when reported (e.g., AAV9, not simply AAV). Distinguish from adenovirus (Ad), which is a different, larger DNA virus vector.
Usage
Usage note: Define at first use. Serotype must be specified when relevant: 'AAV9' not just 'AAV'. 'rAAV' (recombinant AAV) is common in manufacturing contexts. Do not confuse with adenovirus (Ad), which is structurally and immunologically distinct.
In Context
- "AAV9 was selected for its ability to cross the blood–brain barrier and transduce neurons in vivo." — Methods section
- "The cargo size limitation of AAV necessitated the use of a split-intein strategy to deliver the full-length Cas9." — Results section