base editing
A CRISPR-derived technique that converts one DNA base to another at a targeted site without introducing a double-strand break or requiring a donor template.
Full Definition
Base editing uses a catalytically impaired Cas protein (nickase or dead Cas9) fused to a deaminase enzyme to chemically convert a specific nucleotide base—for example, cytosine to uracil (read as thymine) or adenine to inosine (read as guanine)—within a defined editing window. Unlike classical CRISPR-Cas9, base editing does not create double-strand breaks, reducing indel formation and improving precision. Editors should write it as two words ('base editing'), not as a hyphenated or single compound. Subtypes include cytosine base editors (CBEs) and adenine base editors (ABEs).
Usage
Usage note: Two words, no hyphen: 'base editing'. Distinguish from prime editing, which can make all types of point mutations and small indels without requiring DSBs. CBE and ABE subtypes should be defined at first use.
In Context
- "Base editing was employed to correct the point mutation in the beta-globin gene without inducing double-strand breaks." — Results section
- "The author conflated base editing with prime editing; the distinction was flagged for clarification." — Peer review note