Immunofluorometric Assay
Also written as: IFMA — Immunofluorometric Assay
A time-resolved fluorescence-based immunoassay using lanthanide chelates for enhanced sensitivity and reduced interference.
Full Definition
An immunofluorometric assay (IFMA) is a specialized type of fluorescence immunoassay that utilizes lanthanide chelates (typically europium or terbium) as labels to achieve time-resolved fluorescence measurement. The lanthanide labels have unique fluorescence properties, including long fluorescence lifetimes and large Stokes shifts, which allow for time-gated detection that eliminates background fluorescence and non-specific signals. This technology provides exceptional analytical sensitivity and is particularly valuable for measuring low-concentration hormones such as thyroglobulin, intact parathyroid hormone, and tumor markers. IFMA platforms are widely used in automated hormone analyzers and offer superior precision compared to conventional fluorescence assays.
Usage
Usage note: DELFIA is a specific trademarked IFMA platform; use generic term when appropriate.
In Context
- "The thyroglobulin measurement utilizes an immunofluorometric assay for enhanced analytical sensitivity." — Laboratory procedure manual
- "IFMA technology allows detection of intact PTH at concentrations below 1 pg/mL." — Technical specification document