Immunometric Assay
Laboratory technique using excess antibodies to measure hormone concentrations, offering high specificity and sensitivity.
Full Definition
Immunometric assays employ multiple antibodies in excess to capture and quantify specific hormones in biological samples. Unlike competitive immunoassays, immunometric assays use a 'sandwich' approach where the analyte is bound between capture and detection antibodies, allowing for greater specificity and wider dynamic ranges. This methodology is particularly important for measuring low-concentration hormones and peptide hormones. Clinical editors should understand that immunometric assays are less susceptible to matrix interference but may be affected by heterophile antibodies or high-dose hook effects in certain circumstances.
Usage
Usage note: Distinguish from competitive immunoassays in technical documentation.
In Context
- "The TSH measurement was performed using a third-generation immunometric assay." — Laboratory methodology description
- "Immunometric assays provide superior analytical performance for most peptide hormones." — Laboratory comparison study