time-resolved fluoroimmunoassay
Laboratory technique using delayed fluorescence measurement to detect specific analytes in newborn screening specimens.
Full Definition
Time-resolved fluoroimmunoassay (TRFIA) is an analytical method that employs lanthanide chelates as fluorescent labels to measure specific proteins or hormones in dried blood spots. The technique measures fluorescence after a time delay, eliminating background interference and providing highly sensitive and specific results. TRFIA is commonly used in newborn screening for conditions such as congenital hypothyroidism and congenital adrenal hyperplasia, offering superior analytical performance compared to conventional immunoassays.
Usage
Usage note: Hyphenate when used as an adjective; may be abbreviated as TRFIA in technical contexts.
In Context
- "The laboratory implemented time-resolved fluoroimmunoassay to improve thyroid screening sensitivity." — Laboratory validation report
- "Quality control data demonstrated excellent precision for the time-resolved fluoroimmunoassay method." — QC documentation