elution

Full Definition

Elution is the laboratory technique used to dissolve and extract target compounds from dried blood spot specimens for biochemical analysis. The process typically involves adding a specific volume of extraction buffer or methanol to punch discs, followed by incubation and agitation to release amino acids, acylcarnitines, or other metabolites. Proper elution is critical for accurate quantitative results in newborn screening assays.

Usage

Usage note: Distinguish from general chemistry 'elution' when used in newborn screening context.

In Context

• "The elution protocol requires 30-minute incubation at room temperature with gentle shaking." — Standard operating procedure
• "Incomplete elution may result in falsely low analyte concentrations and potential false negatives." — Quality control document