next-generation sequencing
High-throughput DNA sequencing technology enabling rapid analysis of multiple genes, exomes, or whole genomes simultaneously.
Full Definition
Next-generation sequencing (NGS) encompasses massively parallel sequencing technologies that can analyze millions of DNA fragments simultaneously, dramatically reducing cost and time compared to traditional Sanger sequencing. In precision oncology, NGS enables comprehensive tumor profiling through targeted gene panels, whole exome sequencing, or whole genome sequencing. NGS applications include mutation detection, copy number analysis, structural variant identification, and RNA expression profiling. The technology has revolutionized cancer genomics by making routine molecular profiling feasible in clinical practice.
Usage
Usage note: NGS is the standard abbreviation; avoid confusion with specific sequencing platforms or applications.
In Context
- "Next-generation sequencing identified actionable mutations in 68% of the patient cohort." — Clinical study results
- "The laboratory implemented next-generation sequencing for routine tumor profiling." — Clinical laboratory update