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Intermediate Technical IVT

Masson trichrome stain

Pronunciation: mas-SOHN TRY-krom stayn

Histochemical staining technique that differentiates collagen (blue) from muscle tissue (red) in cardiac pathology specimens.

Full Definition

Masson trichrome stain is an essential histochemical technique in cardiac pathology that provides differential coloration of tissue components. In this three-color staining method, collagen and fibrotic tissue appear bright blue, muscle fibers stain red, and nuclei appear dark blue or black. This stain is particularly valuable for assessing myocardial fibrosis, identifying areas of scarring, and evaluating the extent of connective tissue deposition in various cardiomyopathies. The technique uses three different dyes applied sequentially, with the final result highlighting the architectural relationship between myocytes and supporting connective tissue matrix.

Usage

Usage note: Always capitalize 'Masson' as it is an eponym. Specify the color findings when describing results.

In Context

  • "Masson trichrome stain revealed extensive blue-staining interstitial fibrosis between the myocardial fibers." — Histopathology description
  • "The extent of cardiac scarring was best appreciated on Masson trichrome stain sections." — Cardiac biopsy report

Also known as

trichrome stain Masson stain

Don't confuse with

Van Gieson stain Sirius red stain

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